Application of anti-human IgG monoclonal antibodies to antiglobulin reagent. Production and characterization of anti-human IgG monoclonal antibodies.
نویسندگان
چکیده
منابع مشابه
Measurement of Affinity Constant of Anti-human IgG Monoclonal Antibodies by an ELISA-based Method
Background: The affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as ELISA. Affinity of most IgG specific MAbs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
متن کاملproduction and characterization of mouse monoclonal antibodies recognizing human pan-igg specific conformational or linear epitopes
background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...
متن کاملmeasurement of affinity constant of anti-human igg monoclonal antibodies by an elisa-based method
background: the affinity of an antibody to its antigen is a crucial parameter in its biological activity and performance of an immunoassay such as elisa. affinity of most igg specific mabs are often determined by methods which require labeling of either antigen or antibody, and are sometimes difficult to control, do not always lead to the expected signal and often result in immunological modifi...
متن کاملproduction and characterization of mouse monoclonal antibodies recognizing multiple subclasses of human igg
different igg subclass profiles are produced in response to different antigenic stimuli in a variety of diseases. igg subclass levels may reflect disease severity. quantification of igg subclasses depends on the availability of specific monoclonal antibodies (mabs). in the present study seven hybridoma clones producing mabs reactive with multiple subclasses of human igg were established. spleno...
متن کاملProduction of human monoclonal IgG antibodies against Rhesus (D) antigen.
An Epstein-Barr virus (EBV)-transformed human B-cell line ( LB4r ) producing anti-Rhesus [Rho(D) antigen] antibody was fused with a non-immunoglobulin-producing mouse-human heteromyeloma ( SHM - D33 ) and selected in hypoxanthine/aminopterin/thymidine medium containing 0.5 microM ouabain. Surviving hybrids found to secrete specific anti-Rho(D) antibody were cloned by limiting dilution. Two clon...
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ژورنال
عنوان ژورنال: Journal of the Japan Society of Blood Transfusion
سال: 1992
ISSN: 1883-8383,0546-1448
DOI: 10.3925/jjtc1958.38.536